祖源树 Login Scientific Ancients Papers

Multidisciplinary analysis reveals the genetic origins, dietary structure and living conditions of Ming Great Wall residents in Shaanxi, China

HRA018063 Original Paper / DOI2026-08-082 samples (study)

AI interpretationAI-generated

Ming Great Wall soldiers at Qingping Fort: local Yellow River roots, millet diet, hard lives.

This study examined two middle-aged men buried at Qingping Fort, a Ming Dynasty military stronghold in Shaanxi. Using ancient DNA, isotopes and bone analysis, researchers found they ate mainly millet (C4 plants) with little meat, and both had healed nasal fractures, hinting at violence. Their DNA and strontium isotopes point to origins in the Yellow River Basin, with no Eastern steppe ancestry. The authors stress the sample is tiny, so conclusions are preliminary and more research is needed.

Sample interpretation

Only two male samples were analyzed, both from the Ming Dynasty at Qingping Fort. Y-chromosome haplogroups were resolved for both: one N-F1206 and one O-ACT133. Mitochondrial DNA lineages were D4a6 and D4b2b6. These paternal and maternal lines are common in present-day East Asian, especially Chinese, populations. The small number means we cannot generalize to all fort residents, but it offers a first genetic glimpse of Ming frontier soldiers.

For genealogy enthusiasts

For ancestry enthusiasts, this shows that at least some Ming Great Wall residents had deep local Yellow River roots, not steppe origins. It reminds us that frontier populations were diverse and that ancient DNA from military sites can reveal personal stories of diet, health and migration, even with tiny samples.

Abstract

The border region, which lies between different regimes, is highly regarded because of its diverse population, dietary habits, and living conditions. The population around the Great Wall was particularly intriguing during the Ming Dynasty, but it remains unstudied archaeologically. The study uses a multidisciplinary approach that includes paleopathology, isotope analysis, and ancient DNA analysis to investigate two individuals found in a Ming Dynasty military fortress at Qingping Fort. Both individuals were middle-aged males. Pathological examinations revealed nasal bone fractures in both individuals, possibly indicating violent interactions within their group or small-scale inter-group conflict. Carbon isotope analysis indicated that their diet predominantly consisted of C4 plants, which reflected their dietary pattern of millet consumption. Nevertheless, the low δ¹⁵N values implied limited meat intake. The paleopathological evidence, in conjunction with these findings, suggests that the individuals lived in a harsh environment. Based on the analysis of ancient DNA and strontium isotopes, it seems that the individuals are most likely from the Yellow River Basin, and there is no evidence of Eastern steppe ancestry. These findings tentatively suggest that at least some residents of Qingping Fort during the Ming dynasty had close genetic connections with Central Plains populations. Given the small sample size, our conclusions are preliminary, and they emphasize the necessity for future studies with an expanded sample. This work offers a starting point for comprehending the lives of residents in Ming frontier societies and underscores the significance of integrating archaeology with a multidisciplinary approach.

Samples & Data on TheYtree

2samples on site
2Y haplogroup resolved

Paternal (Y-DNA) Haplogroups

HaplogroupSamples
N-F1206 1
O-ACT133 1

Maternal (mtDNA) Haplogroups

Sample Highlights More samples →

SampleY-DNAmtDNACulture / Period
M2 O-ACT133 D4b2b6 明代 Ming· 明代 Ming
M3 N-F1206 D4a6 明代 Ming· 明代 Ming