Peqi'in Cave ancient DNA shows Chalcolithic culture arose from population mixture.
This study analyzed 22 individuals from Peqi'in Cave in Israel, dating to the Late Chalcolithic (4500–3900 BCE). They formed a homogeneous population with mixed ancestry: about 57% from local Levant Neolithic groups, 17% from Iran Chalcolithic-related groups, and 26% from Anatolian Neolithic-related groups. This suggests that migration and mixing of peoples likely drove the cultural changes seen in the southern Levant during this period.
All 22 individuals were from Peqi'in Cave, Israel, and culturally associated with the Late Chalcolithic period. Y-chromosome haplogroups were resolved for 10 males, dominated by T lineages (T-Y4119, T-L208, T-CTS2214, T-L162) plus one E-M123. Mitochondrial DNA included J2a2d, T2e, R0a, K1a-C150T, T1, and I6. This genetic profile reflects a mix of Near Eastern ancestries, consistent with the modeled population mixture.
For ancestry enthusiasts, this study shows that ancient DNA can reveal how population mixing shaped cultural shifts. It highlights the complex ancestry of Levantine Chalcolithic people, with contributions from local, Iranian, and Anatolian Neolithic sources, and warns against assuming direct descent to later groups.
| Sample | Y-DNA | mtDNA | Culture / Period |
|---|---|---|---|
| I1160 | T-CTS2214 | I6a | Late Chalcolithic· 4500–3800 BCE |
| I1178 | T-Y4119 | I6 | Late Chalcolithic· 4500–3800 BCE |
| I1171 | E-M123 | K1a-C150T | Late Chalcolithic· 4500–3800 BCE |
| I1155 | T-L208 | K1a-C150T | Late Chalcolithic· 4500–3800 BCE |
| I1165 | T-CTS2214 | HV1a'b'c | Late Chalcolithic· 4500–3800 BCE |
| I1166 | T-Y4119 | R0a | Late Chalcolithic· 4500–3800 BCE |
| I1170 | T-Y4119 | T1a2 | Late Chalcolithic· 4500–3800 BCE |
| I1172 | T-L208 | K1a18 | Late Chalcolithic· 4500–3800 BCE |
| I1180 | T-L208 | T2-C150T | Late Chalcolithic· 4500–3800 BCE |
| I1187 | T-L162 | N1b | Late Chalcolithic· 4500–3800 BCE |